BS EN ISO 7932:2010 is an international standard that specifies the method for enumeration of presumptive Lactobacillus spp. This technical document provides guidelines on how to determine the number of these bacteria present in a sample using microbiological techniques.
The Importance of BS EN ISO 7932:2010
The enumeration of Lactobacillus spp. is essential in various industries, including food and pharmaceuticals. These bacteria are commonly found in fermented foods and play a crucial role in their production. They contribute to the desirable flavors, textures, and preservation of such products. Additionally, Lactobacillus spp. have been linked to several health benefits, such as boosting the immune system and improving digestion.
Methods Outlined in BS EN ISO 7932:2010
BS EN ISO 7932:2010 provides clear instructions on how to conduct the enumeration of Lactobacillus spp. The standard involves the preparation of serial dilutions of the sample, followed by inoculation onto selective agar plates. After incubation, colonies that exhibit typical Lactobacillus morphology are counted, and the results are expressed as colony-forming units per gram or milliliter (CFU/g or CFU/mL) of the original sample.
Benefits and Limitations of BS EN ISO 7932:2010
The adoption of BS EN ISO 7932:2010 brings several benefits. It ensures standardization and harmonization of methods used for Lactobacillus spp. enumeration globally, facilitating accurate comparison of results between different laboratories and countries. This improves product quality control and consumer safety. However, it is important to note that this standard may have limitations, as it is specific to presumptive Lactobacillus spp. enumeration and may not cover all species or strains within this group. Complementary methods may be required for a comprehensive analysis.
Nina She